Mutagenesis of haploid cultured frog cells
From 13th international congress of genetics; Berkeley, California (20 Aug 1973). Haploid cells afford an opportunity to test some of the assumptions from bacterial genetics which have been adopted by somatic cell geneticists. Haploid cultured cell lines derived from the grass frog Rana pipiens were compared to diploid cell lines in order to test a model which predicts that recessive mutations will be expressed in diploid cells with a frequency equal to the square of that in haploid cells. Haploid and diploid monolayer cultures were compared for (1) survival after exposure to compounds known to be mutagenic for bacteria (a measure of the frequency with which lethal mutations are expressed), and (2) the induction of drug-resistant variants (putative mutants) by such compounds. The proportion of cells that survived from diploid cultures was no more than ten times that from haploid cultures, a much smaller difference than predicted. Furthermore, the frequency of drug-resistant variants was independent of ploidy. (auth)
- Research Organization:
- Institute for Cancer Research, Philadelphia, Pa. (USA)
- NSA Number:
- NSA-29-007429
- OSTI ID:
- 4390780
- Report Number(s):
- COO--3110-10; CONF-730829--1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
*AMINO ACIDS-- GENETIC EFFECTS
*ANIMAL CELLS-- MUTAGENESIS
*EMS-- GENETIC EFFECTS
*NITRO COMPOUNDS-- GENETIC EFFECTS
*NITROSO COMPOUNDS-- GENETIC EFFECTS
*ORGANIC NITROGEN COMPOUNDS-- GENETIC EFFECTS
CELL CULTURES
EMBRYOS
FROGS
MUTATIONS
N48000* --Life Sciences
PLOIDY
SURVIVAL TIME