Cell-mediated mutagenesis and cell transformation of mammalian cells by chemical carcinogens. [Rats, hamsters]

Conference · Fri Dec 31 23:00:00 EST 1976

OSTI ID:6850501

Huberman, E; Langenbach, R

We have developed a cell-mediated mutagenesis assay in which cells with the appropriate markers for mutagenesis are co-cultivated with either lethally irradiated rodent embryonic cells that can metabolize carcinogenic hydrocarbons or with primary rat liver cells that can metabolize chemicals carcinogenic to the liver. During co-cultivation, the reactive metabolites of the procarcinogen appear to be transmitted to the mutable cells and induce mutations in them. Assays of this type make it possible to demonstrate a relationship between carcinogenic potency of the chemicals and their ability to induce mutations in mammalian cells. In addition, by simultaneously comparing the frequencies of transformation and mutation induced in normal diploid hamster cells by benzo(a)pyrene (BP) and one of its metabolites, it is possible to estimate the genetic target size for cell transformation in vitro.

• Research Organization: Oak Ridge National Lab., Tenn. (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6850501
• Report Number(s): CONF-771248-1
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOASSAY
BIOLOGICAL EFFECTS
BODY
CARCINOGENS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
EMBRYONIC CELLS
GLANDS
HAMSTERS
HYDROCARBONS
IRRADIATION
LETHAL IRRADIATION
LIVER
MAMMALS
METABOLISM
MUTAGENESIS
MUTATIONS
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
VERTEBRATES