Comparison of benzo(a)pyrene metabolism and mutation induction in CHO cells using rat liver homogenate (S9) or Syrian hamster embryonic cell-mediated activation systems

Conference · Wed Dec 31 23:00:00 EST 1980

OSTI ID:5962661

Chen, D J; Okinaka, R T; Strniste, G F

Mutagenesis in CHO cells has been studied by the addition of an ezymatically active liver homogenate (S9) fraction. However, the metabolism of procarcinogens, such as benzo(a)pyrene (B(a)P), by rat liver homogenate differs from that in intact cellular activation systems. Consequently, B(a)P-induced mutation frequencies in mammalian cells may vary when different activation systems are used. This study attempts to compare B(a)P metabolism and conjugation in rat liver homogenate (S9 preparation) and in Syrian hamster embryonic (SHE) cells. Furthermore, a CHO mutation assay incorporating either of the activation systems is being used to measure the mutation induction frequency.

• Research Organization: Los Alamos National Lab., NM (USA)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 5962661
• Report Number(s): LA-UR-81-3203; CONF-811086-1; ON: DE82002350
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BODY
CELL CULTURES
COMPARATIVE EVALUATIONS
CONDENSED AROMATICS
DIGESTIVE SYSTEM
GLANDS
HAMSTERS
HYDROCARBONS
LIVER
MAMMALS
METABOLISM
MUTAGEN SCREENING
MUTATION FREQUENCY
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
SCREENING
VERTEBRATES