Cell-mediated mutagenesis by chemical carcinogens

Conference · Sun Jan 01 04:00:00 EST 1978

OSTI ID:5087274

Huberman, E; Langenbach, R

The cell-mediated mutation system, with the proper choice of metabolizing cells, can be used to detect the mutagenic activities of different classes of chemical carcinogens. When fibroblastic cells were used as the metabolizing cells, a correlation between the in vivo carcinogenic activity and the in vitro mutagenic activity of 11 aromatic polycyclic hydrocarbons was observed. When primary liver cells were used as the metabolizing cells, three known liver carcinogens were demonstrated to be mutagenic by the cell-mediated assay, while two non-carcinogenic analogues were not mutagenic. These results from the cell-mediated system suggest that the reactive intermediates of the carcinogens are stable enough to be transferred from the metabolizing cells to the V79 cells. The cell-mediated mutagenesis system is a simple in vitro assay which may simulate the in vivo situation. It was concluded that this approach could be extended to the co-cultivation of cells from other organs or tissues with mutable mammalian cells.

• Research Organization: Oak Ridge National Lab., Tenn. (USA)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5087274
• Report Number(s): CONF-780227-4
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AROMATICS
BIOASSAY
BIOLOGICAL EFFECTS
BODY
CARCINOGENS
CELL CULTURES
DIGESTIVE SYSTEM
GLANDS
HYDROCARBONS
IN VIVO
LIVER
METABOLISM
MUTAGENESIS
MUTAGENS
ORGANIC COMPOUNDS
ORGANS
POLYCYCLIC AROMATIC HYDROCARBONS