Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Characterization of new radiation-sensitive mutant, Escherichia coli K-12 radC102

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576153· OSTI ID:6847840
; ;
A new radiation-sensitive mutant, radC, has been isolated. The radC gene is located at 81.0 min on the Escherichia coli K-12 linkage map. The radC mutation sensitized cells to uv radiation, but unlike most DNA repair mutations, sensitization to X rays was observed only for rich medium-grown cells. For cells grown in rich medium, the radC mutant was normal for ..gamma.. radiation mutagenesis, but showed less uv-radiation mutagenesis than the wild-type strain; it showed normal amount of X- and uv-radiation-induced DNA degradation, and it wasapprox. =60% deficient in recombination ability. The radC strain was normal for host cell reactivation of ..gamma.. and uv-irradiated bacteriophage the radC mutation did not sensitize a recA strain, but did sensitize a radA and a polA strain to X and uv radiation and a uvrA strain to uv radiation. Therefore, it is suggested that the radC gene product plays a role in the growth medium-dependent, recA gene-dependent repair of DNA single-strand breaks after X irradiation, and in postreplication repair after uv irradiation.
Research Organization:
Stanford Univ. School of Medicine, CA
OSTI ID:
6847840
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 97:3; ISSN RAREA
Country of Publication:
United States
Language:
English

Similar Records

Characterization of an Escherichia coli mutant (radB101) sensitive to. gamma. and uv radiation, and methyl methanesulfonate
Journal Article · Mon Feb 28 23:00:00 EST 1983 · Radiat. Res.; (United States) · OSTI ID:5638926
recA (Srf) suppression of recF deficiency in the postreplication repair of UV-irradiated Escherichia coli K-12
Journal Article · Fri Oct 31 23:00:00 EST 1986 · J. Bacteriol.; (United States) · OSTI ID:6829136
Amplified RNase H activity in Escherichia coli B/r increases sensitivity to ultraviolet radiation
Journal Article · Wed Dec 31 23:00:00 EST 1986 · Genetics; (United States) · OSTI ID:6637244

Related Subjects

550400 -- Genetics
550700 -- Microbiology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DATA
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
EXPERIMENTAL DATA
INFORMATION
IONIZING RADIATIONS
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
ULTRAVIOLET RADIATION
X RADIATION