Characterization of an Escherichia coli mutant (radB101) sensitive to. gamma. and uv radiation, and methyl methanesulfonate

Journal Article · Tue Mar 01 04:00:00 EST 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576026· OSTI ID:5638926

Sargentini, N J; Smith, K C

After N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis of Escherichia coli K-12 (xthA14), an X-ray-sensitive mutant was isolated. This sensitivity is due to a mutation, radB101, which is located at 56.5 min on the E.coli K-12 linkage map. The radB101 mutation sensitized wild-type cells to ..gamma.. and uv radiation, and to methyl methanesulfonate. When known DNA repair-deficient mutants were ranked for their ..gamma..-radiation sensitivity relative to their uv-radiation sensitivity, their order was (starting with the most selectively ..gamma..-radiation-sensitive strain): recB21, radB101, wild type, polA1, recF143, lexA101, recA56, uvrD3, and uvrA6. The radB mutant was normal for ..gamma..- and uv-radiation mutagenesis, it showed only a slight enhancement of ..gamma..- and uv-radiation-induced DNA degradation, and it was approx. 60% deficient in recombination ability. The radB gene is suggested to play a role in the recA gene-dependent (Type III) repair of DNA single-strand breaks after ..gamma.. irradiation and in postreplication repair after uv irradiation for the following reasons: the radB strain was normal for the host-cell reactivation of ..gamma..- and uv-irradiated bacteriophage lambda; the radB mutation did not sensitize a recA strain, but did sensitize a polA strain to ..gamma.. and uv radiation; the radB mutation sensitized a uvrB strain to uv radiation.

• Research Organization: Stanford Univ. School of Medicine, CA
• OSTI ID: 5638926
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 93:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550700 -- Microbiology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DATA
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
ESTERS
EXPERIMENTAL DATA
GAMMA RADIATION
INFORMATION
IONIZING RADIATIONS
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTANTS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
STRAND BREAKS
SULFONIC ACID ESTERS
X RADIATION