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Retardation of nodular growth by Ca/sup 2 +/ in the rat liver initiated by DENA and promoted by choline devoid diet (CD)

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6846557
; ;
Male, Fischer 344 rats exposed to a CD diet developed 50% hepatocellular carcinoma. Some of the liver aberrations which develop within 1 week are fat accumulation (8-12 hrs), nuclear lipid peroxidation (24 hrs), DNA damage (48 hrs), cell proliferation (2-3 days), liver cell death (6 days) and mitochondrial lipid peroxidation (5-6 days). The finding that excess Ca/sup 2 +/ (3 times the amount present in CD diet) prevents lipid peroxidation, DNA damage and cell death but not fat accumulation encouraged us to explore whether Ca/sup 2 +/ can also influence the promoting phase of carcinogenesis due to CD feeding. Male, Fischer-344 rats of body weight 110-120 g were initiated with DENA (200 mg per kg body weight i.p.). After a 1 week recovery period the rats were exposed to either CD, or CD + Ca/sup 2 +/ diet. They were killed at 1, 2 and 9 weeks after the start of the special diets. The results indicated that Ca/sup 2 +/ supplementation resulted in a retardation in number and size of ..gamma..-GT positive nodules. Ca/sup 2 +/ supplemented group exhibited only 10 nodules/sq cm compared to 30/sq cm in unsupplemented CD group. Inhibitory action of Ca/sup 2 +/ on the CD-induced liver cell death and proliferation might be one reason for the growth retardation of nodules in Ca/sup 2 +/ supplemented groups.
Research Organization:
Univ. of Toronto, Ontario
OSTI ID:
6846557
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
Country of Publication:
United States
Language:
English

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Related Subjects

550900 -- Pathology
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CALCIUM COMPOUNDS
CARCINOGENESIS
CARCINOMAS
CATIONS
CELL PROLIFERATION
CHARGED PARTICLES
CHOLINE
DIGESTIVE SYSTEM
DISEASES
DRUGS
GLANDS
HYDROXY COMPOUNDS
IONS
LIPOTROPIC FACTORS
LIVER
MAMMALS
NEOPLASMS
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
QUATERNARY COMPOUNDS
RATS
RODENTS
VERTEBRATES