Reactive free radical generation in vivo in heart and liver of ethanol-fed rats: correlation with radical formation in vitro

Journal Article · Mon Nov 30 23:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.24.9223· OSTI ID:6464629

Reinke, L A; Lai, E K; DuBose, C M; McCay, P B

Rats fed a high-fat ethanol-containing diet for 2 weeks were found to generate free radicals in liver and heart in vivo. The radicals are believed to be carbon-centered radicals, were detected by administering spin-trapping agents to the rats, and were characterized by electron paramagnetic resonance spectroscopy. The radicals in the liver were demonstrated to be localized in the endoplasmic reticulum. Rats fed ethanol in a low-fat diet showed significantly less free radical generation. Control animals given isocaloric diets without ethanol showed no evidence of free radicals in liver and heart. When liver microsomes prepared from rats fed the high-fat ethanol diet were incubated in a system containing ethanol, NADPH, and a spin-trapping agent, the generation of 1-hydroxyethyl radicals was observed. The latter was verified by using /sup 13/C-substituted ethanol. Microsomes from animals fed the high-fat ethanol-containing diet had higher levels of cytochrome P-450 than microsomes from rats fed the low-fat ethanol-containing diet. The results suggest that the consumption of ethanol results in the production of free radicals in rat liver and heart in vivo that appear to initiate lipid peroxidation.

• Research Organization: Univ. of Oklahoma Health Sciences Center, Oklahoma City (USA)
• OSTI ID: 6464629
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:24; ISSN PNASA
• Country of Publication: United States
• Language: English

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560300* -- Chemicals Metabolism & Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKYL RADICALS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBON 13
CARBON ISOTOPES
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHEMICAL REACTIONS
DIET
DIGESTIVE SYSTEM
ELECTRON SPIN RESONANCE
ETHANOL
ETHYL RADICALS
EVEN-ODD NUCLEI
GLANDS
HEART
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIVER
MAGNETIC RESONANCE
MAMMALS
MICROSOMES
NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDATION
RADICALS
RATS
RESONANCE
RODENTS
STABLE ISOTOPES
VERTEBRATES