Effect of endotoxin on lipid peroxidation in vivo in selenium and vitamin E deficient rats

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5456235

Sword, J T; Pope, A L; Hoekstra, W G

The authors have used respiratory ethane production by selenium (Se) and vitamin E (E) deficient rats, an index of lipid peroxidation, to identify oxidant stressors which might precipitate sudden tissue degeneration in deficient animals. Other studies have suggested that endotoxin (gram-negative bacterial lipopolysaccharide-LPS) might be such an oxidant stressor, especially in the lungs. Male weanling rats were fed a Se and E deficient diet for about 80 days. Rats were injected ip with Salmonella typhimurium LPS (.25, .5, or 1.0 mg/kg) or saline, and respiratory ethane was collected for 16 hr. In a representative experiment, mean rate of ethane production (nm/100g/hr) was increased (p < .01) by LPS: saline, .48 +/- .04 (SEM); .25 mg LPS/kg, 1.30 +/- .17; .5, 1.47 +/- .18 and 1.0, 1.68 +/- .18. E. coli and S. minnesota LPS gave similar results. Rats fed a supplemented diet (.2 ppm Se and 200 IU E/kg diet) produced less (p < .01) ethane: saline, .068 +/- .009 and .5 mg LPS/kg, .114 +/- .01. Over all experiments LPS produced a small yet significant increase in ethane in rats receiving Se or E supplementation but produced a marked increase in unsupplemented rats. In further studies with LPS treated rats, Se supplementation alone was 73%, and E supplementation alone 99% as effective as Se + E. These results showed that LPS stimulates lipid peroxidation in Se and E deficient rats and that infections may initiate oxidative cell damage in deficient animals. E was more protective than Se against LPS-induced peroxidation.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 5456235
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism &amp; Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ANIMALS
ANTIGENS
BACTERIA
BIOLOGICAL EFFECTS
DIET
ELEMENTS
ENDOTOXINS
ESCHERICHIA COLI
ETHANE
HYDROCARBONS
LIPIDS
MAMMALS
MATERIALS
MICROORGANISMS
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
RATS
RESPIRATION
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SELENIUM
SEMIMETALS
TOXIC MATERIALS
TOXINS
VERTEBRATES
VITAMIN E
VITAMINS