Cellular uptake of {sup 212}BiOCl by Ehrlich ascites cells: A dosimetric analysis

Conference · Fri Jan 01 04:00:00 EST 1999

OSTI ID:684485

Roeske, J C; Whitlock, J L; Harper, P V; Rotmensch, J ^[1]; Stinchcomb, T G ^[2]; Schwartz, J L ^[3]; Hines, J J ^[4]

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1. Univ. of Chicago, IL (United States)
2. DePaul Univ., Chicago, IL (United States). Dept. of Physics
3. Univ. of Washington, Seattle, WA (United States). Dept. of Radiation Oncology
4. Argonne National Lab., IL (United States). Chemistry Div.

Bi-212 is an alpha-emitting radionuclide being investigated as a therapeutic agent in the intraperitoneal treatment of micrometastatic ovarian carcinoma. In evaluating a new therapeutic modality, cell-survival studies are often used as a means of quantifying the biological effects of radiation. In this analysis, Ehrlich ascites cells were irradiated under conditions similar to therapy in various concentrations of Bi-212. Immediately following irradiation, a cell survival assay was performed in which cells were plated and colonies were counted after 10--14 days. Both a macrodosimetric and a microdosimetric approach were used in analyzing these data. These models used as input the fraction of activity within the cell and in solution, the distribution of cell sizes, and the variation of LET along individual alpha-particle tracks. The results indicate that the energy deposited within the nucleus varies significantly among individual cells. There is a small fraction of cell nuclei which receive no hits, while the remaining cells receive energy depositions which can differ significantly from the mean value. These dosimetric parameters are correlated with measured cell survival and will be a useful predictor of outcome for therapeutic doses.

• Research Organization: Oak Ridge Associated Universities, TN (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• OSTI ID: 684485
• Report Number(s): ORISE--99-0164-Vol.1; CONF-960536--PROC.-Vol.1; ON: DE99002903
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ASCITES TUMOR CELLS
BISMUTH 212
CARCINOMAS
DOSIMETRY
LET
OVARIES
RADIATION DOSES
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
RADIOTHERAPY