Production of chromosome aberrations in various mammalian cells by triethylenemelamine
The cytogenetic effects of triethylenemelamine (TEM) were studied using five different mammalian tissues. Treatments of 0.1 and 0.2 mg/kg TEM on differentiating mouse spermatogonia and bone marrow cells showed no significant differences in the frequency of chromosomal aberrations produced in these two tissues. At higher doses, however, the sensitivities of the two tissues appear to be different. The frequency of aberrations varies with time after treatment, with the greatest amount occurring at the later fixation times. Results of an experiment on primary spermatocytes indicated a correlation between the frequency of chromosome aberrations and DNA replication. Human peripheral leukocytes were utilized in an attempt to clarify the cell stage specificity of TEM-induced chromosome aberrations. Cultures were treated with TEM prior to PHA stimulation (G/sub 0/), as well as various time intervals after stimulation (late G/sub 1/, S, and G/sub 2/). The most sensitive stages of the cell cycle to aberration induction were later G/sub 1/ and S, with chromatid aberrations the predominant type. A very low yield of chromosome damage was observed with the G/sub 0/ and G/sub 1/ treated stages. The experiments described tend to support the view that TEM is most effective at inducing aberrations when an intervening round of DNA replication has occurred.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 6844653
- Journal Information:
- Genetics; (United States), Vol. 88:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Role of base damage in aberration formation: interaction of aphidicolin and x-rays
Study of the mechanisms involved in the increased sensitivity of mouse myeloid leukemic cells to the induction of chromosome aberrations
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKYLATING AGENTS
BIOLOGICAL EFFECTS
BONE MARROW CELLS
CHROMOSOMAL ABERRATIONS
DNA REPLICATION
LEUKOCYTES
SPERMATOCYTES
SPERMATOGONIA
CELL CYCLE
MAN
MICE
ANIMAL CELLS
ANIMALS
ANTIMITOTIC DRUGS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DRUGS
GAMETES
GERM CELLS
MAMMALS
MUTATIONS
NUCLEIC ACID REPLICATION
PRIMATES
RODENTS
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550301 - Cytology- Tracer Techniques