Study of the mechanisms involved in the increased sensitivity of mouse myeloid leukemic cells to the induction of chromosome aberrations

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6694938

Aardema, M E.J.

X ray-induced chromosome aberrations were analyzed in in vitro normal mouse RFM bone marrow cells and in an RFM mouse myeloid leukemia cell line, ML-1. Chromosome aberrations were analyzed in cells that were irradiated in G/sub 1/ of the cell cycle. Dicentrics and terminal deletions were induced in greater frequencies in ML-1 cells than in normal cells, and chromatid-type aberrations were induced only in ML-1 cells. The induction of chromatid-type aberrations in ML-1 and not in normal cells suggested: (1) a difference between the normal and ML-1 cells in cell cycle progression after irradiation and/or (2) a difference between the cells in the repair of X ray-induced DNA damage. Flow cytometric analyses showed that cells of both cell types in G/sub 2/ at the time of irradiation were similarly delayed in progression into mitosis. However, normal cells in G/sub 1/ were delayed in progression into and through the S phase while the ML-1 cells progressed without delay into the S phase. The X ray-induced delay in G/sub 1/ normal cells may serve as a protective mechanism allowing extra time for DNA repair before DNA replication in the S phase. It was concluded that the lack of a cell cycle delay after irradiation in G/sub 1/ ML-1 cells allowed cells to progress into the S phase with unrepaired DNA damage where a misrepair event results in the formation of chromatid-type aberrations.

• Research Organization: Tennessee Univ., Knoxville (USA)
• OSTI ID: 6694938
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BONE MARROW CELLS
CELL CULTURES
CELL CYCLE
CELL FLOW SYSTEMS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
GROWTH
HEMIC DISEASES
IN VITRO
IONIZING RADIATIONS
LEUKEMIA
MAMMALS
MICE
MITOTIC DELAY
MUTATIONS
MYELOID LEUKEMIA
NEOPLASMS
RADIATIONS
RODENTS
SOMATIC CELLS
VERTEBRATES
X RADIATION