Mechanisms of chromosomal aberration production. I. Aberration induction by ultraviolet light
Chromosomal aberration production was studied in V-79 Chinese hamster tissue culture cells by uv light administered during the post-DNA-synthetic G/sub 2/ phase of the cell cycle. The treatment produced achromatic lesions ond some chromatid deletions in the first post-irradiation mitosis, but no isochromatid deletions or chromatid exchange oberrations. In contrast, when G/sub 2/ uv- irradiated cells were examined in their second postirradiation mitosis, there were significant yields of chromatidtype aberrations of all types, includlng isochromatid deletions and chromatid exchanges. It was earlier reported that uv- irradiation during the pre-DNA-synthetic G/sub 1/ phase of the cell cycle induces only chromatid aberrations and also that most chromosomal oberration production by uv in G/sub 1/ can be photoreactivated in cells possessing the photoreactivating enzyme. A model is presented for chromosomal aberration production by uv. In the model all aberration production is enzymatically modiated, a consequence of the functioning of known molecular repair mechanisms. (HLW)
- Research Organization:
- Vanderbilt Univ., Nashville
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-29-021113
- OSTI ID:
- 4351949
- Journal Information:
- Mutat. Res., v. 20, no. 3, pp. 387-402, Other Information: Orig. Receipt Date: 30-JUN-74
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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