Alloantigen-activated lymphocytes from mice bearing a spontaneous nonimmunogenic adenocarcinoma inhibit its growth in vivo by recruiting host immunoreactivity

Journal Article · Fri Nov 01 04:00:00 EST 1985 · J. Immunol.; (United States)

OSTI ID:6840789

Giovarelli, M; Santoni, A; Forni, G

Nylon wool columns eluting lymphocytes from the spleen of mice bearing a clinically evident spontaneous, nonimmunogenic adenocarcinoma of recent origin (TS/A) do not display cytotoxic response, release of lymphokines, and proliferation in vitro against TS/A cells, nor do they inhibit TS/A tumor growth in a Winn-type neutralization assay in vivo. After 5-day co-culture with allogeneic spleen cells from mice differing at multiple minor histocompatibility antigens only, these lymphocytes are still noncytolytic against TS/A cells, whereas they release interferon-..gamma.., mediate delayed-type hypersensitivity (DTH) reactions, and inhibit TS/A tumor growth in the Winn assay. In the Winn test, alloactivated lymphocytes from TS/A tumor-bearing mice are more effective than those from normal mice on a per cell basis. The induction of this TS/A tumor inhibition ability depends on the presence in the cultures of Thy-1/sup +/ lymphocytes. The presence of Lyt-2/sup +/ lymphocytes is also important, whereas that of asialo GM1/sup +/ is not. The TS/A inhibition in vivo by alloactivated lymphocytes mostly depends on Thy-1/sup +/, Lyt-2/sup -/ and asialo GM/sup -/ lymphocytes, even though a few Thy/sup -/ cells are also very efficient tumor inhibitors. The alloactivated lymphocytes inhibit TS/A tumor growth by recruiting the radiosensitive effector mechanisms of the recipient mice required for ultimate tumor rejection. TS/A tumor rejection leaves a specific DTH and an immunologic memory resulting in rejection of a second lethal TS/A challenge in a significant number of mice.

• Research Organization: Univ. of Turin, Italy
• OSTI ID: 6840789
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 135:5; ISSN JOIMA
• Country of Publication: United States
• Language: English

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