Chromosome specific repetitive DNA sequences
Patent
·
OSTI ID:6834064
This patent describes a method for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family members and consensus sequences of the repetitive DNA families for the chromosome preferential sequences. The selected low homology regions are then hybridized with chromosomes to determine those low homology regions hybridized with a specific chromosome under normal stringency conditions. In accordance with the invention, oligomers are provided which are useful in forming a probe for specific chromosome identification under normal stringency conditions.
- Assignee:
- US Dept. of Energy, Washington, DC (United States)
- Patent Number(s):
- A; US 5064948
- Application Number:
- PPN: US 7-155451
- OSTI ID:
- 6834064
- Country of Publication:
- United States
- Language:
- English
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