An oligonucleotide probe specific to the centromeric region of human chromosome 5

Journal Article · Wed Dec 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80387-8· OSTI ID:7200810

Matera, A G; Ward, D C ^[1]; Baldini, A ^[2]

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1. Yale Univ. School of Medicine, New Haven, CT (United States)
2. Baylor College of Medicine, Houston, TX (United States)

The authors have designed a 28-mer alpha satellite oligonucleotide probe that, at high stringency, hybridizes specifically to the centromeric region of human chromosome 5. Using fluorescence in situ hybridization they also find that, under low-stringency conditions, this oligomer hybridizes to chromosomes 1, 5, and 16. The hybridization to chromosome 16 was somewhat unexpected since clonally derived probes from this alphoid DNA subset hybridize to chromosomes 1, 5, and 19 at high stringency. They also compare and contrast the sequences of previously cloned alphoid DNAs from chromosomes 5, 16, and 19 in the region corresponding to the oligomer. This oligonucleotide should facilitate the genomic and cytogenetic analysis of human chromosome 5.

• OSTI ID: 7200810
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 18:3; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
DNA
DNA HYBRIDIZATION
GENETIC MAPPING
HUMAN CHROMOSOME 1
HUMAN CHROMOSOME 16
HUMAN CHROMOSOME 19
HUMAN CHROMOSOME 5
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS