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Title: Chromosome specific repetitive DNA sequences

Abstract

A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).

Inventors:
 [1];  [1]
  1. (Los Alamos, NM)
Publication Date:
Research Org.:
Los Alamos National Laboratory (LANL), Los Alamos, NM
OSTI Identifier:
868065
Patent Number(s):
US 5064948
Assignee:
United States of America as represented by Department of Energy (Washington, DC) LANL
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
chromosome; specific; repetitive; dna; sequences; method; provided; determining; nucleotide; useful; forming; probe; identify; chromosomes; preferably; situ; hybridization; cell; embodiment; preferential; determined; library; recombinant; clones; families; identified; homology; sequence; respectively; belong; variant; selecting; pattern; genomic; dissimilar; shown; respective; selected; family; classified; nonspecific; sequenced; regions; result; contract; department; energy; w-7405-eng-36; dna sequences; dna sequence; nucleotide sequences; nucleotide sequence; recombinant dna; chromosome specific; situ hybridization; repetitive sequences; identify specific; specific chromosomes; specific chromosome; /999/435/436/

Citation Formats

Moyzis, Robert K., and Meyne, Julianne. Chromosome specific repetitive DNA sequences. United States: N. p., 1991. Web.
Moyzis, Robert K., & Meyne, Julianne. Chromosome specific repetitive DNA sequences. United States.
Moyzis, Robert K., and Meyne, Julianne. Tue . "Chromosome specific repetitive DNA sequences". United States. https://www.osti.gov/servlets/purl/868065.
@article{osti_868065,
title = {Chromosome specific repetitive DNA sequences},
author = {Moyzis, Robert K. and Meyne, Julianne},
abstractNote = {A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {1}
}

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