Metabolism, mass spectral analysis and mode of action of trichothecene mycotoxins. Final report, 15 July 1985-14 July 1989

Technical Report · Fri Dec 22 04:00:00 EST 1989

OSTI ID:6832996

Mirocha, C J; Pawlosky, R J; Gunther, R; Abbas, H K

Methods of analysis for T-2 toxin, HT-2 and T-2-tetraol in blood and urine were developed using hybrid tandem mass spectrometry, more specifically, Multiple Reaction Monitoring (MRM). Essentially, the mass spectra of the above toxins were obtained in electron impact, the fragments studied for selection of appropriate parent and daughters were generated with the objective of using them analytically. As an example, m/z 478 of the trifluoroacetate derivative of T-2 toxin was reacted in the collision chamber (field free region three) with argon and 23 eV to produce daughters 12, 138 and 180. These were used in method development so that T-2 was detected in a biological matrix with a sensitivity of 1 part per billion. A field method of urine collection was developed for the analysis of T-2 toxin. An attempt was made to find toxic isolates of Fusarium in soils of the Arctic of Norway that would explain some of the hemorrhagic activity noted with this genus. More specifically, descriptions of toxicity of biological warfare agents originating in Southeast Asia included extreme hemorrhaging. To this end, toxic isolates were found that caused extreme hemorrhaging in rats. The natural product responsible for the toxicity was isolated, purified and characterized as wortmannin. Wortmannin was shown to cause hemorrhaging in the heart, bladder, stomach and thymus. The chemistry, NMR and mass spectra of wortmannin are presented.

• Research Organization: Minnesota Univ., St. Paul, MN (USA). Dept. of Plant Pathology
• OSTI ID: 6832996
• Report Number(s): AD-A-218488/5/XAB
• Country of Publication: United States
• Language: English

Related Subjects

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
450600* -- Military Technology
Weaponry
& National Defense-- Chemical & Biological-- (1990)
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BIOLOGICAL WARFARE AGENTS
BIOLOGICAL WASTES
BLADDER
BLOOD
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
DIGESTIVE SYSTEM
DOCUMENT TYPES
EUMYCOTA
EUROPE
FUNGI
FUSARIUM
GASTROINTESTINAL TRACT
HEART
HEMORRHAGE
LYMPHATIC SYSTEM
MAMMALS
MASS SPECTRA
MATERIALS
METABOLISM
NMR SPECTRA
NORWAY
ORGANS
PARASITES
PATHOLOGICAL CHANGES
PLANTS
PROGRESS REPORT
RATS
RODENTS
SCANDINAVIA
SOILS
SPECTRA
STOMACH
SYMPTOMS
THYMUS
TOXIC MATERIALS
TOXICITY
TOXINS
URINARY TRACT
URINE
VERTEBRATES
WASTES
WEAPONS
WESTERN EUROPE