Metabolic fate of tritium-labeled T-2 toxin, a trichothecene mycotoxin, in swine

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6702755

Corley, R A

The metabolic fate of T-2 toxin was determined in two female crossbred swine following the intravascular administration of one millicurie of tritium-labeled T-2 toxin at a nonlethal dose of 0.15 mg/kg body weight. The plasma elimination phase half-life was 90 minutes for total tritium residues. A total of 13.1 and 1.3 percent of the administration dose was found in the gall bladders in addition to 17.9 and 42.5% in the urine in the two pigs, S1 and S2, respectively, 4 hours after dosing. Free metabolites, identified by thin-layer chromatography, represented less than 20 and 30% of the metabolite residues in bile and urine, respectively, with the parent compound, T-2 toxin, never exceeding 0.25%. The major free metabolites were 3-OH HT-2 and T-2 triol. Glucuronide conjugates represented 63 and 77% of the metabolite residues in urine and bile, respectively. The major conjugated metabolites were glucuronides of HT-2, 3'-OH T-2, 3'-OH HT-2 and T-2 toxin. Neosolaniol, 4-deacetyl-neosolaniol and T-2 tetraol were also identified in addition to 3 unknown metabolites. In the tissues, the greatest amount of radioactivity was located in the gastrointestinal tract (15.5 and 24.1% of the dose for the 2 pigs, S1 and S2, respectively). The remaining tissues sampled accounted for approximately 5% of the dose for the 2 pigs. Twenty-one metabolites were identified in tissues following reverse phase HPLC radiochromatography.

• Research Organization: Illinois Univ., Urbana (USA)
• OSTI ID: 6702755
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
BILE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD-PLASMA CLEARANCE
BODY FLUIDS
CHROMATOGRAPHY
CLEARANCE
DOMESTIC ANIMALS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MATERIALS
METABOLISM
REACTION KINETICS
SEPARATION PROCESSES
SWINE
THIN-LAYER CHROMATOGRAPHY
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
VERTEBRATES
WASTES