Electrophoretic analysis of myofibrillar and mitochondrial proteins from ventricular myocardium from normal and copper deficient rats

Journal Article · Wed Feb 08 23:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:6832880

McCormick, R J; Ovecka, G D; Medeiros, D M ^[1]

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1. Univ. of Wyoming, Laramie (USA)

Male Long-Evans rats were fed copper adequate (8 mg Cu/kg diet, N=9) or copper deficient (0.4 mg Cu/kg diet, N=10) diets from weanling until sacrificed (9 weeks). Animals fed the copper deficient diet had decreased hematocrit and liver copper levels and increased heart weight verifying copper deficiency. Left ventricle cardiac tissue was evaluated for changes in myofibrillar and mitochondrial proteins by gradient polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and densitometry. No differences in individual myofibrillar proteins as a function of copper deficiency were apparent. In the mitochondrial protein fraction 2 peptides (approx. molecular weights 22,000 and 19,000) present in normal ventricles were absent from copper deficient, severely hypertrophic tissues of present in lesser amounts in copper deficient animals exhibiting a lesser degree of hypertrophy. The molecular weight of these peptides corresponds closely to published weights of cytochrome C oxidase subunits. The changes observed here may represent alterations in translation or assembly of cytochrome C oxidase as a function of copper deficiency.

• OSTI ID: 6832880
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (USA) Vol. 3:3; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARDIOVASCULAR SYSTEM
COPPER
CYTOCHROME OXIDASE
DIET
ELECTROPHORESIS
ELEMENTS
ENZYMES
HAEM DEHYDROGENASES
HEART
HYPERTROPHY
MAMMALS
METALS
MUSCLES
MYOCARDIUM
NUTRITIONAL DEFICIENCY
ORGANS
OXIDOREDUCTASES
PATHOLOGICAL CHANGES
PROTEIN STRUCTURE
RATS
RODENTS
TISSUES
TRANSITION ELEMENTS
VERTEBRATES