Radioimmunoassay of propranolol

Journal Article · Mon Aug 01 04:00:00 EDT 1983 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00260a028· OSTI ID:6832563

Eller, T D; Knapp, D R; Walle, T

A series of specific antibodies against d,I-propranolol has been developed by conjugation of the novel 5'-, 7'-, and 8'-(3-aminopropoxy)propranolol derivatives to succinylated bovine serum albumin. By preservation of an intact side chain in the antigen, significantly improved specificity to parent drug vs. inactive side chain metabolites was demonstrated compared to previous methods. The radioimmunoassay (RIA) based on these antibodies is capable of detecting as little as 20 pg of propranolol in a toluene extract of tissue or plasma. Samples run in parallel with gas chromatography/mass spectrometry measurements showed excellent agreement between the two (GC/MS) methods in both dogs and humans. Comparison of direct plasma assay of human samples with GC/MS analysis indicated elevated results by RIA due to high levels of an unknown conjugate of 4'-hydroxypropranolol which is eliminated by the extraction. This immunoassay should provide a useful and simplified alternative to GC/MS in the quantitation of propranolol in biological samples. 1 figure, 1 table.

• Research Organization: Univ. of South Carolina, Charleston
• OSTI ID: 6832563
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 55:9; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DATA
DOGS
EXPERIMENTAL DATA
GAS CHROMATOGRAPHY
HYDROCARBONS
HYDROXY COMPOUNDS
IMMUNE SERUMS
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
MAN
MASS SPECTRA
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
SENSITIVITY
SEPARATION PROCESSES
SPECTRA
TOLUENE
TRACER TECHNIQUES
VERTEBRATES