A specific radioimmunoassay for osteocalcin with advantageous species crossreactivity

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(82)90309-8· OSTI ID:6725825

Patterson-Allen, P; Brautigam, C E; Grindeland, R E; Asling, C W; Callahan, P X

The specificities of immunoassays to rat and bovine osteocalcin are examined. Extracts of noncalcified tissues and tissue fractions are unreactive to the antibody, with the exception of the kidney, in which the reactive component appears to be identical with osteocalcin by gel filtration and dose dilution analysis. The assays, developed against protein isolated from bone, are also demonstrated to be reactive to the native protein (bone in situ) and to osteocalcin in serum. The assays are sensitive to less than 50 pg osteocalcin. Intra- and interassay coefficients of variation are less than 6.8%. The bovine antibody crossreacts with human, horse, monkey, baboon, and cat osteocalcin, while the rat antibody crossreacts with dog and mouse.

• OSTI ID: 6725825
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 120:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BODY
BONE TISSUES
CATTLE
CONNECTIVE TISSUE
DOMESTIC ANIMALS
ISOTOPE APPLICATIONS
KIDNEYS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RATS
RODENTS
RUMINANTS
TISSUES
TRACER TECHNIQUES
VERTEBRATES