Enzyme immunoassay for rat growth hormone: applications to the study of growth hormone variants

Journal Article · Mon Jun 29 04:00:00 EDT 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90068-3· OSTI ID:6409664

Farrington, M A; Hymer, W C

A sensitive and specific competitive enzyme immunoassay (EIA) for rat growth hormone was developed. In this assay soluble growth hormone and growth hormone adsorbed to a solid-phase support compete for monkey anti-growth hormone antibody binding sites. The immobilized antibody-growth hormone complex is detected and quantified using goat anti-monkey immunoglobin G covalently conjugated to horse radish peroxidase. Therefore, a high concentration of soluble growth hormone in the sample will result in low absorbance detection from the colored products of the enzyme reaction. Assay parameters were optimized by investigating the concentration of reagents and the reaction kinetics in each of the assay steps. The assay can be performed in 27 hours. A sensitivity range of 0.19 ng to 25 ng in the region of 10 to 90% binding was obtained. Near 50% binding (3 ng) the intraassay coefficient of variation (CV) was 5.54% and the interassay CV was 5.33%. The correlation coefficient (r/sup 2/) between radioimmunoassay and EIA was 0.956 and followed the curve Y = 0.78X + 1.0. 9 references, 6 figures.

• Research Organization: Pennsylvania State Univ., University Park
• OSTI ID: 6409664
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 40:26; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
DATA
ENZYME IMMUNOASSAY
EXPERIMENTAL DATA
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
REACTION KINETICS
REAGENTS
RECEPTORS
RODENTS
TRACER TECHNIQUES
VERTEBRATES