Polyamines as mediators of insulin's action on pyruvate dehydrogenase, /sup 45/Ca/sup 2 +/ fluxes, and membrane transport

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6832102

Goldstone, A D; Koenig, H; Lu, C Y

Insulin (IN) induces a rapid stimulation of Ca/sup 2 +/ fluxes and membrane transport in mouse kidney cortex which involves rapid polyamine synthesis. 1.3 nM (IN) induced an early (<30 sec) stimulation of pyruvate dehydrogenase (PDH) activity which peaked at 2 min (1.5-fold) followed by a significant decline (-31%) below basal between 5 and 10 min. IN induced a similar biphasic effect on membrane transport, with stimulation of ..cap alpha..-aminoisobutarate, 2-deoxyglucose and horseradish peroxidase transport peaking at 1 min, and decreasing below basal at 5-10 min. IN stimulation of ornithine decarboxylase (ODC) activity, polyamine accumulation and /sup 45/Ca/sup 2 +/ influx and efflux peaked at 1-2 min and returned to basal levels by 5-10 min. The ODC inhibitor ..cap alpha..-difluoromethylornithine (DFMO, 5 mM) abolished IN stimulation of PDH, /sup 45/Ca/sup 2 +/ fluxes and membrane transport, and putrescine (.5 mM) nullified DFMO inhibition. IN (50 mUnits/kg) in rats induced an early (< 1 min), coordinate increase in liver polyamine levels and PDH activity which peaked at 6 min and then declined. These effects were blocked by DFMO (200 mg/kg), and putrescine (100 mg/kg) negated DFMO inhibition. These results indicate that newly synthesized polyamines mediate IN's effects on PDH activity, Ca/sup 2 +/ fluxes, and membrane transport.

• Research Organization: Northwestern Univ. Medical School, Chicago, IL
• OSTI ID: 6832102
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
KINETICS
MAMMALS
MEMBRANE TRANSPORT
MICE
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
RADIOISOTOPES
REACTION KINETICS
RODENTS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES