Renal ornithine decarboxylase activity, polyamines, and compensatory renal hypertrophy in the rat

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6834383

Humphreys, M H; Etheredge, S B; Lin, Shanyan; Ribstein, J; Marton, L J ^[1]

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1. San Francisco General Hospital, CA (USA) Univ. of California, San Francisco (USA)

The authors determined the role of ornithine decarboxylase (ODC) in compensatory renal hypertrophy (CRH) by relating renal ODC activity and polyamine content to kidney size, expressed as a percent of body weight, 1 wk after unilateral nephrectomy (UN). In normal rats, renal ODC activity increased after UN; 1 wk later the remaining kidney weight had increased. Renal concentration of putrescine, the product of ODC's decarboxylation of ornithine, was increased 3, 8, and 48 h after UN, but concentrations of polyamines synthesized later in the pathway, spermidine and spermine, were not appreciably affected. Pretreatment with difluoromethylornithine (DFMO), an irreversible inhibitor of ODC inhibited both base-line renal ODC activity and putrescine concentration as well as increases stimulated by UN, although concentrations of spermidine and spermine were not decreased. In hypophysectomized rats, both increased renal ODC activity and CRH occurred as well, indicating that these two consequences of UN do not require intact pituitary function. Thus stimulation of renal ODC activity and putrescine content do not appear critical to the process of CRH after UN.

• OSTI ID: 6834383
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:2; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
BODY
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
DECARBOXYLASES
ENZYME ACTIVITY
ENZYMES
HYPERTROPHY
HYPOPHYSECTOMY
KIDNEYS
LYASES
MAMMALS
MEDICINE
NEPHRECTOMY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
ORNITHINE
PATHOLOGICAL CHANGES
PUTRESCINE
RATS
RODENTS
SPERMIDINE
SPERMINE
SURGERY
SYNTHESIS
VERTEBRATES