Polyamines mediate abnormal Ca/sup 2 +/ transport and Ca/sup 2 +/-induced cardiac cell injury in the calcium paradox
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6958406
Ca/sup 2 +/-free perfusion renders heart cells Ca/sup 2 +/-sensitive so that readmission of Ca/sup 2 +/ causes a sudden massive cellular injury attributed to abnormal entry of Ca/sup 2 +/ into cells (Ca paradox). Hormonal stimulation of Ca/sup 2 +/ fluxes was earlier shown to be mediated by polyamines (PA). 5 min perfusion of rat heart with Ca/sup 2 +/-free medium induce a prompt 40-50% decline in levels of the PA putrescine (PUT), spermidine and spermine and their rate-regulatory synthetic enzyme ornithine decarboxylase (ODC), and readmission of Ca/sup 2 +/-containing medium abruptly (< 30-60 sec) increased the levels of ODC and PA. The ODC inhibitor ..cap alpha..-difluoromethylornithine (DFMO, 5mM) blocked Ca/sup 2 +/ reperfusion-induced increases in ODC and PA and also prevented increased /sup 45/Ca/sup 2 +/ uptake and heart injury, manifested by loss of contractility, release of enzymes (CPK, LDH), myoglobin and protein, and E.M. lesions (contracture bands, mitochondrial changes). 1 mM PUT negated DFMO inhibition, repleted heart PA and restored Ca/sup 2 +/ reperfusion-induced /sup 45/Ca/sup 2 +/ influx and cell injury. These data indicate that the Ca/sup 2 +/-directed depletion-repletion cycle of ODC and PA triggers excessive transsarcolemmal Ca/sup 2 +/ transport leading to the calcium paradox.
- Research Organization:
- Northwestern Univ. Medical School, Chicago, IL
- OSTI ID:
- 6958406
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Polyamines as mediators of insulin's action on pyruvate dehydrogenase, /sup 45/Ca/sup 2 +/ fluxes, and membrane transport
Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes
Ornithine decarboxylase and extracellular polyamines regulate microvascular sprouting and actin cytoskeleton dynamics in endothelial cells
Conference
·
Tue Mar 04 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6832102
Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes
Journal Article
·
Tue Oct 31 20:00:00 EDT 2023
· Cell Reports Medicine
·
OSTI ID:2332845
Ornithine decarboxylase and extracellular polyamines regulate microvascular sprouting and actin cytoskeleton dynamics in endothelial cells
Journal Article
·
Fri Oct 01 00:00:00 EDT 2010
· Experimental Cell Research
·
OSTI ID:22209907
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HEART
INJURIES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE TRANSPORT
NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HEART
INJURIES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE TRANSPORT
NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
VERTEBRATES