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Sensitive method to quantify the terminal differentiation of cultured epidermal cells

Journal Article · · Exp. Cell Res.; (United States)
; ;

Terminal differentiation of normal and malignant keratinocytes is routinely determined by the ability of these cells to form cornified envelopes after incubation with a calcium ionophore. The authors have used the human squamous cell carcinoma, SqCC/YI, to quantify cellular differentiation by the formation of detergent-insoluble protein. The methodology developed employs the metabolic labeling of detergent-insoluble cellular protein with (/sup 35/S)methionine in the presence of a calcium ionophore. The ratio of filter-retainable radioactivity to that of total cellular protein was shown to be closely correlated to the results obtained by measuring the number of envelope-competent cells when cells were induced to enter a pathway of terminal differentiation in culture by serum deprivation or by treatment with hydrocortisone, and during the inhibition of maturation by either retinoic acid (RA) or epidermal growth factor (EGF). This way of measuring the degree of terminal differentiation of epidermal cells is a relatively simple one that readily allows the simultaneous measurement of multiple samples.

Research Organization:
Yale Univ. School of Medicine, New Haven, CT
OSTI ID:
6831718
Journal Information:
Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 167:1; ISSN ECREA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CARCINOMAS
CELL DIFFERENTIATION
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EPIDERMIS
EPITHELIUM
ESTERS
EVEN-ODD NUCLEI
GROWTH
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
NEOPLASMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RETINOIC ACID
SKIN
SULFUR 35
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
TUMOR CELLS