Effects of retinoids on differentiation, lipid metabolism, epidermal growth factor, and low-density lipoprotein binding in squamous carcinoma cells

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90174-1· OSTI ID:6621957

Ponec, M; Weerheim, A ^[1]; Havekes, L ^[2]; Boonstra, J ^[3]

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1. University Hospital, Leiden (Netherlands)
2. Gaubius Institute TNO, Leiden (Netherlands)
3. Univ. of Utrecht (Netherlands)

The relationship among keratinocyte differentiation capacity, lipid synthesis, low-density lipoprotein (LDL) metabolism, plasma membrane composition, and epidermal growth factor (EGF) binding has been studied in SCC-12F2 cells. The differentiation capacity of the cells, i.e., ionophore-induced cornified envelope formation, was inhibited by various retinoids and stimulated by hydrocortisone. Retinoids that caused a significant reduction of cornified envelope formation, i.e., retinoic acid and 13-cis-retinoic acid, caused only minor changes in lipid synthesis and plasma membrane composition. Arotinoid ethylsulfone, having a minor effect on cornified envelope formation, caused a drastic inhibition of cholesterol synthesis resulting in changes in the plasma membrane composition. Hydrocortisone stimulated cornified envelope formation but had only minor effects on lipid synthesis and plasma membrane composition. Of all retinoids tested, only arotinoid ethylsulfone caused a drastic increase in EGF binding, while hydrocortisone had no effect. These results clearly demonstrate that the plasma membrane composition is not related to keratinocyte differentiation capacity, but most likely does determine EGF binding. Furthermore, EGF binding does not determine keratinocyte differentiation capacity.

• OSTI ID: 6621957
• Journal Information: Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 171:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANIMAL TISSUES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACID ESTERS
CELL CONSTITUENTS
CELL DIFFERENTIATION
CELL MEMBRANES
CELL PROLIFERATION
CORTICOSTEROIDS
EPITHELIUM
ESTERS
GLUCOCORTICOIDS
GROWTH FACTORS
HYDROCORTISONE
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LIPIDS
LIPOPROTEINS
MEMBRANES
METABOLISM
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RADIORECEPTOR ASSAY
RETINOIC ACID
SKIN
STEROIDS
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
VITAMIN A
VITAMINS