In vitro comparisons of SENCAR and BALB/c primary epidermal cells

Journal Article · Mon Sep 01 00:00:00 EDT 1986 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.866839· OSTI ID:6985013

Strickland, J E; Allen, P T; Sauder, D N; Kawamura, H; Fong, M C; Yuspa, S H

Grafting experiments show that the enhanced sensitivity of the SENCAR mouse to skin carcinogenesis by initiation and promotion is a property of the skin itself, suggesting the usefulness of in vitro studies to elucidate the mechanism. Such studies have indicated that cultured epidermal cells of SENCAR mice and the resistant BALB/c strain are remarkably similar in a variety of respects. Primary epidermal cells cultured in the presence of various concentrations of 12-O-tetradecanoylphorbol-13-acetate (TPA), retinoic acid, epidermal growth factor (EGF), hydrocortisone, or fluocinolone acetonide failed to reveal differences in growth between BALB/c and SENCAR cells. Cells from these animals bound comparable amounts of EGF with similar kinetics, and the modulation of this binding by TPA and retinoic acid was indistinguishable between strains. Spontaneous expression of infectious, endogenous xenotropic type C RNA virus at very low levels could be demonstrated in primary BALB/c epidermal cells and both BALB/c and SENCAR epidermal lines resistant to Ca/sup 2 +/-induced terminal differentiation. The number of foci of initiated cells after exposure to carcinogens in vivo or in vitro did not differ significantly between SENCAR and BALB/c, suggesting that SENCAR sensitivity is primarily to promotion. However, there are qualitative differences between SENCAR and BALB/c foci. The appearance of foci of cells resistant to terminal differentiation in untreated SENCAR cultures supports the evidence from in vivo studies for the existence of a constitutively initiated cell population in SENCAR mouse skin.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 6985013
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 68; ISSN EVHPA
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CARBOXYLIC ACID ESTERS
CARCINOGENESIS
CARCINOGENS
DNA REPAIR
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
ESTERS
IN VITRO
IONIZING RADIATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MICE
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHORBOL ESTERS
PROMOTERS
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
RETINOIC ACID
RODENTS
SKIN
SPECIES DIVERSITY
TISSUES
TRITIUM COMPOUNDS
TUMOR PROMOTERS
VERTEBRATES
X RADIATION