Host cell reactivation studies with epidermal cells of mice sensitive and resistant to carcinogenesis
Primary epidermal cells from AKR, BALB/c, CD-1, and SENCAR mice, listed in order of least to most sensitive to epidermal carcinogenesis by initiation and promotion protocols, were found to be equally competent to ''reactivate'' herpes simplex virus type 1 irradiated by germicidal ultraviolet radiation. Nontumorigenic BALB/c epidermal cell lines selected in vitro for resistance to terminal differentiation after in vivo or in vitro treatment with initiating doses of carcinogens showed virus survival curves similar to those of primary cells. Similarly, primary cultures which were allowed to grow to confluency following a single treatment with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (100 ng/ml) retained normal host cell reactivation. Host cell reactivation studies with mouse dermal fibroblasts could not be done because of the failure of the herpes simplex virus to infect these cells and produce plaques. These results demonstrate that survival of ultraviolet light-damaged virus in primary epidermal cells in culture is unrelated to whether the cells are derived from mice sensitive or resistant to epidermal carcinogenesis. Furthermore, virus survival is not changed by tumor promoter treatment or by treatment with initiating doses of carcinogens which results in differentiation-resistant cells.
- Research Organization:
- National Cancer Inst., Bethesda, MD
- OSTI ID:
- 6408062
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 44:3; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CARCINOGENESIS
CARCINOGENS
DISEASES
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
HERPES SIMPLEX
HOST-CELL REACTIVATION
INFECTIOUS DISEASES
INHIBITION
MAMMALS
MICE
ORGANS
PATHOGENESIS
PROMOTERS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SENSITIVITY
SKIN
SKIN DISEASES
TISSUES
TUMOR PROMOTERS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES