DNA content determination of micronucleated polychromatic erythrocytes induced by clastogens and spindle poisons in mouse bone marrow and peripheral blood

Conference · Fri Jan 01 04:00:00 EST 1993 · Cytometry (Baltimore); (United States)

OSTI ID:6831587

Grawe, J; Amneus, H ^[1]; Zetterberg, G ^[2]

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1. Swedish Univ. of Agricultural Sciences, Uppsala (Sweden) Uppsala Univ. (Sweden)
2. Uppsala Univ. (Sweden)

The frequencies and DNA distributions of micronuclei in polychromatic erythrocytes from the bone marrow and peripheral blood of mice after four different treatments were determined by flow cytometry. Polychromatic erthrocytes were detected using the fluorescent RNA stain thiazole orange, while micronuclei were detected with the DNA stain Hoechst 33342. The treatments were X-irradiation (1 Gy), cyclophosphamide (30 mg/kg), vincristine sulfphate (0.08 mg/kg), and cochicine (1 mg/kg). All treatments showed increased frequencies of micronucleated polychromatic erythrocytes at 30h after treatment in the bone marrow (colchicine 50h) and at 50h in the peripheral blood. The clostogenic agents X-irradiation and cyclophosphamide and the spindle poisons vincristine sulphate and cochicine could be grouped according to the fluorescent characteristics of the induced micronuclei as well as the relative frequency of small (0.5-2% if the diploid G1 DNA content) and large (2-10%) micronuclei. In the peripheral blood the relative frequency of large micronuclei was lower than in the bone marrow, indicating that they were partly eliminated before entrance into the peripheral circulation. The nature of presumed micronuclei was verified by sorting. The potential of this approach to give information on the mechanism of induction of micronuclei is discussed.

• OSTI ID: 6831587
• Report Number(s): CONF-9303114--
• Conference Information: Journal Name: Cytometry (Baltimore); (United States) Journal Volume: 6
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIGENS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL NUCLEI
CHROMOSOMES
CYTOLOGICAL TECHNIQUES
DETECTION
DNA
ELECTROMAGNETIC RADIATION
ERYTHROCYTES
FLUORESCENCE
HAZARDOUS MATERIALS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
LUMINESCENCE
MALFORMATIONS
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOLOGICAL CHANGES
RADIATION EFFECTS
RADIATIONS
RNA
SORTING
THIAZOLES
TOXIC MATERIALS
TOXINS
X RADIATION