Molecular analysis of a Neurospora crassa gene expressed during conidiation

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.6.2411· OSTI ID:6829861

Roberts, A N; Berlin, V; Hager, K M; Yanofsky, C

The asexual developmental pathway in the life cycle of the filamentous fungus Neurospora crassa culminates in the formation of spores called conidia. Several clones of genomic Neurospora DNA have been isolated that correspond to mRNA species expressed during conidiation and not during mycelial growth. In this paper the authors describe the characterization of one of these clones, named pCon-10a. This clone contains two genes, con-10 and con-13, which are induced coordinately during the late stages of conidiation. The two genes are separated by 1.4 kilobases of DNA; they are located on linkage groups IV and are transcribed from the same strand of DNA. The molecular organization and sequence of one of these genes, con-10, and its flanking regions are presented. Full-length cDNA clones for con-10 were isolated and sequenced, and transcription-initiation and polyadenylation sites were defined. The con-10 also were isolated and sequenced, and transcription-initiation and polyadenylation sites were defined. The con-10 gene contains an open reading frame interrupted by two small introns and encodes an 86-amino-acid residue polypeptide that is both hydrophilic and weakly acidic. Expression of the con-10 gene in various mutants defective at different stages of conidiation indicates that it plays a role after aerial hyphal development. Possible functions, organization, and regulation of conidiation-specific genes are discussed.

• Research Organization: MRC Immunochemistry Unit, Dept. of Biochemistry, Univ. of Oxford South Parks Road, Oxford OX1 3QU (GB)
• OSTI ID: 6829861
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:6; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
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550200* -- Biochemistry
550800 -- Morphology
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
CELL DIFFERENTIATION
CLONING
DNA-CLONING
FUNGI
GENE REGULATION
GENETIC MAPPING
MAPPING
MESSENGER-RNA
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NEUROSPORA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHENOTYPE
PLANTS
PROTEINS
RNA
TRANSCRIPTION