Quantitative response of bone marrow colony-forming units (CFU-C and PFU-C) in weaning beagles exposed to acute whole-body. gamma. irradiation
Using a methylcellulose-supported bone marrow culture system, the dose-response relationships of suspended granulocyte-monocyte colonies (CFU-C) and adherent fibroblastic colonies (PFU-C) were investigated in 2- to 3-month-old beagles exposed to acute whole-body ..gamma.. irradiation. Groups of weanling beagles were exposed at a rate of 140 R/hr delivered from a /sup 60/Co ..gamma.. source achieving total exposures ranging from 0 to 586 R. Twenty-four hours following irradiation, bone marrow was collected, plated into methylcellulose, and after 1 week of incubation both colony types were quantitated. In addition, bone marrow cellularity determinations were made for a variety of bones using an /sup 59/Fe-labeling technique. The results show a D/sub 37/ for the linear part of the slope of 70 R for CFU-C. Although within the limits of the experiment a D/sub 37/ could not be established for the fibroblastic populations, the results indicate a substantial degree of radioresistance for these elements supporting our previous studies on PFU-C, performed on mice, which suggested a D/sub 37/ of approximately 400 R for the progenitors of these fibroblastic elements.
- Research Organization:
- Univ. of California, Davis
- OSTI ID:
- 6817155
- Journal Information:
- Radiat. Res.; (United States), Vol. 74:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW CELLS
BIOLOGICAL RADIATION EFFECTS
CFU
RADIOSENSITIVITY
BEAGLES
CELL CULTURES
COBALT 60
FIBROBLASTS
GAMMA RADIATION
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DOGS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals