Hematological effects: comparative studies on the radiation survival characteristics in vivo and in vitro of bone marrow-derived clonogenic populations (CFU-C and PFU-C) and some observations on bone marrow cellularity in beagles

Technical Report · Sun Jun 01 04:00:00 EDT 1975

OSTI ID:4062525

Wilson, F D; O'Grady, L; Momeni, M; Wheeling, J A; Klein, K; Graham, R; Jow, N; Di Bartola, S; Goldman, M

Data accumulated for the hematological effects program are reviewed. Particular emphasis is given to the effects of acute and chronic irradiation on hematopoietic progenitor populations (CFU-C, colony-forming units in culture) and ''candidate'' mesenchymal progenitors (PFU-C, plaque-forming units in culture) using methylcellulose bone marrow culture systems and both in vivo and in vitro radiation exposure protocols. Preliminary results of studies on the temporal effects of acute x-irradiation on the capacity of PFU-C to generate colony stimulating activity (CSA) are also presented. The results of such experiments are providing the basis upon which future RBE studies on a variety of nuclides will be structured. Data (including age related changes) is also presented on in vivo bone marrow cellularity determinations, as well as for marrow stem cell quantitative studies using nondestructive techniques for normal Beagles. In these studies, two techniques for correction of variable effects of hemodilution are compared. Such studies are also providing baseline data for the $sup 60$Co hematological effects program. (auth)

• Research Organization: California Univ., Davis (USA). School of Veterinary Medicine
• NSA Number: NSA-33-023523
• OSTI ID: 4062525
• Report Number(s): UCD--472-122
• Country of Publication: United States
• Language: English

Related Subjects

*HEMATOPOIETIC SYSTEM-- BIOLOGICAL RADIATION EFFECTS
*RADIOISOTOPES-- RBE
560152* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Vertebrates
ACUTE IRRADIATION
BEAGLES
BONE MARROW
CELL CULTURES
CHRONIC IRRADIATION
IN VITRO
IN VIVO
MICE
N48520* --Life Sciences--Radiation Effects on Animals-- Vertebrates
SURVIVAL TIME
X RADIATION