Radioimmunoassy for phencyclidine (PCP) in serum. [Dogs]
Journal Article
·
· Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:6816903
This accurate, sensitive radioimmunoassay for determining phencyclidine concentrations in serum specimens involves the use of anti-phencyclidine sera, 0.1 mL of serum specimen, an iodinated tracer, and a solid-phase separation. Phencyclidine metabolities do not show significant cross reactivity, but several phencyclidine analogs do cross react. Within-run coefficients of variation for human and dog serum ranged from 2.5 to 13% for concentrations from 2.0 to 500 ..mu..g/L. Day-to-day coefficients of variation for human and dog serum ranged from 4.3 to 16.7% for concentrations ranging from 2.0 to 09.0 ..mu..g/L. The sensitivity of the radioimmunoassay is <0.5 ..mu..g/L. Thirty serum specimens from two dogs given 1 mg of phencyclidine per kilogram body weight were analyzed by radioimmunoassay and a gas-chromatographic method. Nonparametris statistical comparison and linear regression showed that results from the two procedures correlate well (r/sup 2/ = 0.952). Concentration-time data from the two dogs are presented to illustrate the utility of the radioimmunoassay for examining phencyclidine disposition.
- Research Organization:
- Univ. of Arizona, Tucson
- OSTI ID:
- 6816903
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:7; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD SERUM
CENTRAL NERVOUS SYSTEM AGENTS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GAS CHROMATOGRAPHY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
PSYCHOTROPIC DRUGS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
REACTION KINETICS
SENSITIVITY
SEPARATION PROCESSES
TESTING
TRACER TECHNIQUES
VALIDATION
VERTEBRATES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD SERUM
CENTRAL NERVOUS SYSTEM AGENTS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOGS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GAS CHROMATOGRAPHY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
PSYCHOTROPIC DRUGS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
REACTION KINETICS
SENSITIVITY
SEPARATION PROCESSES
TESTING
TRACER TECHNIQUES
VALIDATION
VERTEBRATES