KRAS codon 12 mutations occur very frequently in pancreatic adenocarcinomas
Journal Article
·
· Nucleic Acids Research; (UK)
- State Univ. of Leiden (Netherlands)
DNAs from human pancreatic adenocarcinomas were analyzed for the presence of mutations in codons 12, 13 and 61 of the NRAS, KRAS and HRAS gene. Formalin-fixed and paraffin-embedded tissue was used directly in an in vitro amplification reaction to expand the relevant RAS sequences. The mutations were detected by selective hybridization using mutation-specific synthetic oligonucleotides. In 28 of the 30 patients the authors found a mutation in codon 12 of the KRAS gene. This result confirms findings that KRAS mutations occur frequently in adenocarcinomas of the exocrine pancreas. The mutations are predominantly G-T transversions, in contrast to the KRAS mutations in colon tumors which are mainly G-A transitions. Furthermore, in a portion of the tumors the mutation appears to be homozygous.
- OSTI ID:
- 6810815
- Journal Information:
- Nucleic Acids Research; (UK), Journal Name: Nucleic Acids Research; (UK) Vol. 16:16; ISSN 0305-1048; ISSN NARHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
AROMATICS
AZAARENES
AZINES
BODY
CARCINOMAS
CODONS
DIGESTIVE SYSTEM
DISEASES
DNA BASE TRANSITIONS
DNA HYBRIDIZATION
DNA SEQUENCING
ENDOCRINE GLANDS
GENE AMPLIFICATION
GENE MUTATIONS
GENES
GLANDS
GUANINE
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROXY COMPOUNDS
MUTATIONS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ONCOGENES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PANCREAS
PATIENTS
PURINES
PYRIMIDINES
RIBOSIDES
STRUCTURAL CHEMICAL ANALYSIS
THYMIDINE
59 BASIC BIOLOGICAL SCIENCES
AMINES
AROMATICS
AZAARENES
AZINES
BODY
CARCINOMAS
CODONS
DIGESTIVE SYSTEM
DISEASES
DNA BASE TRANSITIONS
DNA HYBRIDIZATION
DNA SEQUENCING
ENDOCRINE GLANDS
GENE AMPLIFICATION
GENE MUTATIONS
GENES
GLANDS
GUANINE
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
HYDROXY COMPOUNDS
MUTATIONS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ONCOGENES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PANCREAS
PATIENTS
PURINES
PYRIMIDINES
RIBOSIDES
STRUCTURAL CHEMICAL ANALYSIS
THYMIDINE