Frequency and spectrum of c-Ki-ras mutations in human sporadic colon carcinoma, carcinomas arising in ulcerative colitis, and pancreatic adenocarcinoma

Journal Article · Sat Jun 01 04:00:00 EDT 1991 · Environmental Health Perspectives; (United States)

DOI:https://doi.org/10.1289/ehp.919327· OSTI ID:6100012

Burmer, G C; Rabinovitch, P S; Loeb, L A ^[1]

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1. Univ. of Washington School of Medicine, Seattle (United States)

Sporadic colon carcinomas, carcinomas arising in chronic ulcerative colitis, and pancreatic adenocarcinomas have been analyzed for the presence of c-Ki-ras mutations by a combination of histological enrichment, cell sorting, polymerase chain reaction, and direct sequencing. Although 60% (37/61) of sporadic colon carcinomas contained mutations in codon 12, only 1 of 17 specimens of dysplasia or carcinoma from ulcerative colitis patients contained c-Ki-ras mutations, despite a high frequency of aneuploid tumors. In contrast, a higher percentage (16/20 = 80%) of pancreatic adenocarcinomas contained mutations in c-Ki-ras 2, despite a lower frequency of DNA aneuploidy in these neoplasms. Moreover, the spectrum of mutations differed between sporadic colon carcinoma, where the predominant mutation was a G to A transition, and pancreatic carcinomas, which predominantly contained G to C or T transversions. These results suggest that the etiology of ras mutations is different in these three human neoplasms.

• OSTI ID: 6100012
• Journal Information: Environmental Health Perspectives; (United States), Journal Name: Environmental Health Perspectives; (United States) Vol. 93; ISSN 0091-6765; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANEUPLOIDY
BODY
CARCINOMAS
DIGESTIVE SYSTEM
DIGESTIVE SYSTEM DISEASES
DISEASES
DNA BASE TRANSITIONS
ENDOCRINE GLANDS
ETIOLOGY
GENES
GLANDS
MUTATION FREQUENCY
NEOPLASMS
ONCOGENES
ORGANS
PANCREAS
PLOIDY