Glucose cycling is markedly enhanced in pancreatic islets of obese hyperglycemic mice

Journal Article · Tue May 01 04:00:00 EDT 1990 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-126-5-2413· OSTI ID:6808836

Khan, A; Chandramouli, V; Ostenson, C G; Berggren, P O; Loew, H L; Landau, B R; Efendic, S ^[1]

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1. Karolinska Hospital, Stockholm (Sweden)

Pancreatic islets from fed 7-month old lean and obese hyperglycemic mice (ob/ob) were incubated with 3H2O and 5.5 mM or 16.7 mM glucose. Incorporation of 3H into the medium glucose was taken as the measure of glucose-6-P hydrolysis to glucose. Glucose utilization was measured from the yield of 3H2O from (5-3H)glucose. Only 3-4% of the glucose phosphorylated was dephosphorylated by the lean mouse islets irrespective of the glucose concentration. In contrast, the ob/ob mouse islets at 5.5 mM glucose dephosphorylated 18% of the glucose phosphorylated and 30% at 16.7 mM. Thus, the islets of hyperglycemic mice demonstrate increased glucose cycling as compared to the islets of normoglycemic lean mice.

• OSTI ID: 6808836
• Journal Information: Endocrinology; (USA), Journal Name: Endocrinology; (USA) Vol. 126:5; ISSN ENDOA; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
CONNECTIVE TISSUE
DIABETES MELLITUS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
GLUCOSE
HEXOSES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
METABOLIC DISEASES
METABOLISM
MICE
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PANCREAS
PATHOGENESIS
PHOSPHORYLATION
RODENTS
SACCHARIDES
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
WATER