Glucose metabolism and recycling by hepatocytes of OB/OB and ob/ob mice
- Cedars-Sinai Medical Center, Los Angeles, CA (USA)
Hepatocytes were prepared from livers of ob/ob (obese diabetic) mice and their lean (OB/OB) siblings that had been fasted for 24 h. The hepatocytes were incubated with (U-14C, 2-3H)-, (U-14C, 3-3H)-, and (U-14C, 6-3H)glucose at concentrations from 20 to 120 mM. 14C was recovered mainly in CO2, glycogen, and lactate. Tritium was recovered in water and glycogen. The yield in labeled products from (2-3H)glucose ranged from two to three times that from (U-14C)glucose. The yields from (3-3H)- and (6-3H)glucose were similar, and 1.3-1.7 times that from (U-14C)glucose. At 40 mM, total utilization of glucose by obese mice was about twice that for lean mice, but there was little difference at 120 mM. The rate of recycling between glucose and glucose 6-phosphate was calculated. An equation to calculate the rate of recycling of glucose from the 2-3H/U-14C ratio in glycogen is derived in the APPENDIX. Our results show that (1) the utilization of glucose by hepatocytes from obese diabetic mice exceeds that of their lean controls, (2) the rate of glucose phosphorylation in both groups greatly exceeds glucose uptake and the rate of glycogen synthesis, (3) glucose phosphorylation represents a difference between a high glucokinase rate and hydrolysis of glucose 6-phosphate, and (4) recycling of glucose carbon between glucose 6-phosphate and pyruvate occurs within mouse hepatocytes.
- OSTI ID:
- 6422280
- Journal Information:
- American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 259; ISSN 0002-9513; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
The glucose-galactose paradox in neonatal murine hepatic glycogen synthesis
Regulation of lipid synthesis in hepatocytes from lean and obese Zucker rats
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALDEHYDES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CONNECTIVE TISSUE
DIABETES MELLITUS
DISEASES
ENDOCRINE DISEASES
FASTING
GLUCOSE
GLYCOGEN
HEXOSES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
LACTATES
LIVER CELLS
MAMMALS
METABOLIC DISEASES
METABOLISM
MICE
MONOSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
RODENTS
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES