Glucose cycling in islets from healthy and diabetic rats

Journal Article · Sat Mar 31 23:00:00 EST 1990 · Diabetes; (USA)

DOI:https://doi.org/10.2337/diab.39.4.456· OSTI ID:7008064

Khan, A; Chandramouli, V; Ostenson, C G; Loew, H L; Landau, B R; Efendic, S ^[1]

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1. Karolinska Hospital, Stockholm (Sweden)

Pancreatic islets from healthy (control) and neonatally streptozocin-induced diabetic (STZ-D) rats, a model for non-insulin-dependent diabetes mellitus, were incubated with {sup 3}H{sub 2}O and 5.5 or 16.7 mM glucose. At 5.5 mM glucose, no detectable ({sup 3}H)glucose was formed. At 16.7 mM, 2.2 patom.islet-1.h-1 of {sup 3}H was incorporated into glucose by the control islets and 5.4 patom.islet-1.h-1 by STZ-D islets. About 75% of the {sup 3}H was bound to carbon-2 of the glucose. Glucose utilization was 35.3 pmol.islet-1.h-1 by the control and 19.0 pmol.islet-1.h-1 by the STZ-D islets. Therefore, 4.5% of the glucose-6-phosphate formed by the control islets and 15.7% by the STZ-D islets was dephosphorylated. This presumably occurred in the beta-cells of the islets catalyzed by glucose-6-phosphatase. An increased glucose cycling, i.e., glucose----glucose-6-phosphate----glucose, in islets of STZ-D rats may contribute to the decreased insulin secretion found in these animals.

• OSTI ID: 7008064
• Journal Information: Diabetes; (USA), Journal Name: Diabetes; (USA) Vol. 39:4; ISSN 0012-1797; ISSN DIAEA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
DIABETES MELLITUS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
ENZYMES
ESTERASES
GLANDS
GLUCOSE
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
HYDROLASES
INHIBITION
INSULIN
ISOTOPE APPLICATIONS
MAMMALS
METABOLIC DISEASES
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PATHOGENESIS
PEPTIDE HORMONES
PHOSPHATASES
RATS
RODENTS
SACCHARIDES
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES