Ultraviolet radiation induction of ornithine decarboxylase in rat keratinocytes

Journal Article · Tue May 01 04:00:00 EDT 1990 · Cancer Research; (USA)

OSTI ID:6808521

Rosen, C F; Gajic, D; Drucker, D J ^[1]

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1. Women's College Hospital, Toronto, Ontario (Canada)

UV radiation plays an important role in the induction of cutaneous malignancy, including basal cell and squamous cell carcinomas and malignant melanoma. In addition to its effects on DNA damage and repair mechanisms, UV radiation has been shown to modulate the expression of specific genes, altering the levels of their mRNAs and the synthesis of their corresponding proteins. In order to gain further information about the molecular effects of UV radiation, we have studied the regulation of ornithine decarboxylase (ODC) gene expression in response to UVB radiation. ODC is the rate-limiting enzyme in polyamine biosynthesis, is involved in growth and differentiation, and has been implicated in carcinogenesis. Keratinocytes grown in culture were either sham-irradiated or exposed to increasing doses of UVB (1-5 mJ/cm2). Northern blot analysis of keratinocyte RNA under basal conditions demonstrated the presence of two ODC mRNA transcripts. Increasing exposure to UVB resulted in a dose-dependent increase in the levels of both ODC mRNA transcripts. The induction of ODC gene expression following UVB was noted 2 h after UVB exposure, and ODC mRNA levels continued to increase up to 24 h after UVB exposure. The UVB-induced increase in ODC gene expression was not serum dependent, despite the ability of serum alone to induce ODC gene expression. The mRNA transcripts for actin and hexosaminidase A were not induced after UVB exposure. These studies show that the UVB-induced increase in ODC activity is due, at least in part, to an increase in ODC gene expression and they provide a useful model for the analysis of the molecular effects of UVB radiation.

• OSTI ID: 6808521
• Journal Information: Cancer Research; (USA), Journal Name: Cancer Research; (USA) Vol. 50:9; ISSN 0008-5472; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
DECARBOXYLASES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ENZYME INDUCTION
ENZYMES
GENE REGULATION
KERATIN
LYASES
MAMMALS
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORNITHINE
PROTEINS
RADIATION EFFECTS
RADIATIONS
RATS
RNA
RODENTS
SCLEROPROTEINS
TRANSCRIPTION
ULTRAVIOLET RADIATION
VERTEBRATES