Cyclobutane-type pyrimidine photodimer formation and induction of ornithine decarboxylase in human skin fibroblasts after UV irradiation

Journal Article · Thu Dec 01 04:00:00 EST 1988 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12477095· OSTI ID:6485947

Niggli, H J; Roethlisberger, R

Cyclobutane-type pyrimidine photodimers as well as the induction of ornithine decarboxylase (ODC) may serve as biochemical markers of the mutagenic and carcinogenic effects of ultraviolet light (UV). For this reason, it is important to compare the formation of pyrimidine dimers with the induction of ODC in human skin fibroblasts after irradiation with UVC (200-290 nm) and UVB (290-320 nm). In our studies we determined cytosine-thymine (C-T) as well as thymine-thymine dimer yields (T-T) by high-pressure liquid chromatography in cultures of neonatal normal human foreskin-derived fibroblasts after irradiation with UVC and UVB light. It was found that the yield of dimerization and the ratio of T-T/C-T decreased from the UVC to the UVB region. Time-course studies of ODC-induction in the same cells indicated that the maximal activity after UVB irradiation was retarded compared to UVC exposure. For the UV-induced ODC-levels, however, no significant difference in maximal induction could be measured after UVC and UVB irradiation at fluences where comparable yields of thymine dimerization are produced. Similar ODC-maxima were obtained with strains from children, while cells from adults showed significantly less pronounced ODC induction, indicating that ODC-response decreases with age and may therefore be used as a marker of aging.

• Research Organization: Cosmital SA, Marly (Switzerland)
• OSTI ID: 6485947
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 91:6; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MARKERS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBON-CARBON LYASES
CARBOXY-LYASES
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DECARBOXYLASES
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INDUCTION
ENZYMES
FIBROBLASTS
GENE REGULATION
LIQUID COLUMN CHROMATOGRAPHY
LYASES
MAMMALS
MAN
ORGANS
PRIMATES
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
SEPARATION PROCESSES
SKIN
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES