DNA damage, photorepair, and survival in fish and human cells exposed to UV radiation
- Brookhaven National Lab., Upton, NY (United States)
The effect of various wavelengths of UVB radiation on the induction of cyclobutane pyrimidine dimers in fish cells and human fibroblasts and the repair of these lesions were studied using an UV-endonuclease to measure dimers (endonuclease sensitive sites) by sedimentation of radioactive DNA, by gel electrophoresis of unlabeled DNA, and by cell survival. The data show that fish cells have an efficient photoreactivation system at wavelength > 304 nm that reverses cytoxicity and dimer formation after exposure to filtered sunlamp irradiation of a shorter wavelength ([lambda] > 290 nm). Shorter wavelengths in UVB (>304 nm) are more effective in photoreversal than longer ones (>320 nm). As a consequence, 50-85% of dimers induced by these wavelengths in fish are photoreactivated while they are being formed. A major cytotoxicological lesion is the cyclobutane pyrimidine dimers. Cultured human fibroblasts do not possess such a repair system. These results indicate that sunlamp irradiation has wavelengths that both damage and repair DNA. 37 refs., 5 figs., 1 tab.
- OSTI ID:
- 7199195
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 22:1; ISSN 0893-6692; ISSN EMMUEG
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
NEAR ULTRAVIOLET RADIATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
REPAIR
ULTRAVIOLET RADIATION