Estimation of kinetic parameters for glucose transport in human brain cortex
3-O-C-11-methyl-D-glucose (CMG), F-18-3-deoxy-3-fluoro D-glucose (3FDG), and dynamic positron-emission-tomography (dPET) were used to measure the rate constants for glucose transport across the blood brain barrier (BBB) in human cortex. The assay takes advantage of CMG or 3FDG being practically not metabolized in brain and being transported back from the tissue into the circulation. The simultaneous registration of tracer concentration in blood and tissue by dPET at 1 min intervals for 40 min yields time activity curves, which permit the in vivo determination of the rate constants for CMG or 3FDG transport across the BBB. In the present study, 4 healthy volunteers and 10 patients suffering from a single-sided ischemic brain disease were examined. In all cases the CMG/3FDG measurements were carried out at two different glucose plasma concentrations i.e. at normoglycemia and hyperglycemia after i.v. application of 10 g glucose. The determination of glucose plasma concentration was performed just before and immediately after the CMG/3FDG study. Using these data and a new mathematical model the Michaelis-Menten constant (K/sub M/) and maximal velocity (V/sub M/) for CMG, 3FDG and glucose transport across the BBB in normal and non-affected human cortex were determined. K/sub M CMG/ was 7.21 ..mu..mol/g; K/sub M 3FDG/ was 3.93 ..mu..mol/g and K/sub M gluc/ was 6.31 ..mu..mol/g. V/sub M/ was found in all cases to be 2.1 ..mu..mol/min g. The data obtained suggest that the CMG/3FDG method might provide a powerful tool for studying the mechanisms involved in the pathological alterations of glucose carrier system.
- Research Organization:
- Inst. of Medicine
- OSTI ID:
- 6795009
- Report Number(s):
- CONF-850611-
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 26:5; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Glucose transport and utilization in the human brain: model using carbon-11 methylglucose and positron emission tomography
3-Fluoro-Deoxyglucose for the assessment of cerebral perfusion and glucose transport - Indications for extracranial-intracranial arterial bypass and followup studies
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANTIMETABOLITES
BIOLOGICAL LOCALIZATION
BLOOD FLOW
BLOOD-BRAIN BARRIER
BODY
BRAIN
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CEREBRAL CORTEX
CEREBRUM
CHEMICAL REACTION KINETICS
COMPUTERIZED TOMOGRAPHY
COUNTING TECHNIQUES
DATA ANALYSIS
DIAGNOSTIC TECHNIQUES
DRUGS
DYNAMIC FUNCTION STUDIES
EMISSION COMPUTED TOMOGRAPHY
FLUORODEOXYGLUCOSE
GLUCOSE
HEXOSES
KINETICS
LABELLING
MATHEMATICAL MODELS
MEMBRANE TRANSPORT
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PERMEABILITY
POSITRON COMPUTED TOMOGRAPHY
RADIOASSAY
RADIOISOTOPE SCANNING
RADIONUCLIDE KINETICS
REACTION KINETICS
SACCHARIDES
TOMOGRAPHY