Effects of epidermal growth factor and platelet-derived growth factor on c-fos and c-myc mRNA levels in normal human fibroblasts

Journal Article · Wed Jul 01 04:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90261-8· OSTI ID:6794494

Paulsson, Y; Bywater, M; Westermark, B ^[1]; Heldin, C H ^[2]

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1. University Hospital, Uppsala (Sweden)
2. Ludwig Institute of Cancer Research, Uppsala (Sweden)

The mRNA levels of two proto-oncogenes, c-fos and c-myc, were determined in human foreskin fibroblasts exposed to epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) in a serum-free, defined medium (MCDB 104). Untreated, quiescent cells were found to have low or undetectable levels of c-fos and c-myc mRNA. Within 10 min after the addition of EGF or PDGF the c-fos mRNA level increased, reached a peak at 30 min, and then declined to the control level after 60 min. The level of c-myc mRNA increased somewhat later and peaked after 8 h in cultures treated with either of the growth factors. The c-myc mRNA level remained elevated throughout the 24 h of investigation. The concentrations of EGF and PDGF required for a maximal effect on c-fos or c-myc expression were found to be similar to those that give maximal effect on cell proliferation. Both c-fos and c-myc mRNA expression were superinduced by the addition of cycloheximide. The present results conform to the view that the c-fos and c-myc proto-oncogenes may be important (or necessary) but not sufficient for the initiation of DNA synthesis. Moreover, the finding that both EGF and PDGF increase c-fos and c-myc expression supports the previous suggestion that these two growth factors may in part act via a common intracellular pathway in the prereplicative phase of human fibroblasts.

• OSTI ID: 6794494
• Journal Information: Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 171:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
CYCLOHEXIMIDE
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DRUGS
EPIDERMIS
EPITHELIUM
FIBROBLASTS
FUNGICIDES
GENES
GROWTH FACTORS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MATERIALS
MESSENGER-RNA
MITOGENS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONCOGENES
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
SKIN
SOMATIC CELLS
TISSUES
TRANSCRIPTION