Induction of C-FOS, C-MYC and P53 by US -adrenergic receptor (US -AR) stimulation of rat parotid acinar cells (RPAC)

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6359603

Kousvelari, E E; Louis, J; Curran, T; Baum, B J

Treatment of rats with the US -agonist isoproterenol (ISO) results in dramatically increased parotid gland protein synthesis, processing and cell proliferation. The authors have shown that in RPAC in vitro, US -AR stimulation has similar effect on protein synthesis and processing. Proto-oncogenes have been implicated in growth regulation, differentiation and in mediating some extracellular stimulated events at the level of gene expression. To understand the regulation of cellular events after US -AR stimulation, the expression of c-fos, c-myc and p53 was investigated. RPAC were incubated with or without 10 VM ISO for 15, 30, 60 min. mRNA was isolated from cells and hybridization analysis was performed on nitrocellulose paper-transferred mRNA using TSP-labeled DNA probes. At early time points, the levels of c-fos gene activation in ISO-treated and control cells were comparable. After 60 min of ISO treatment, a sharp 20-30 fold induction of c-fos expression occurred. Similar increases in c-myc and p53 gene expression were observed after 60 min of ISO treatment. The authors data indicate that early effects of US -AR stimulation of RPAC include induction of c-fos, c-myc and p53 gene expression as well as enhanced protein synthesis and processing.

• Research Organization: National Institute of Health, Bethesda, MD
• OSTI ID: 6359603
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
DRUGS
GENE REGULATION
GENES
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONCOGENES
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RNA
RODENTS
SALIVARY GLANDS
SYMPATHOMIMETICS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES