Induction of C-FOS, C-MYC and P53 by US -adrenergic receptor (US -AR) stimulation of rat parotid acinar cells (RPAC)
Treatment of rats with the US -agonist isoproterenol (ISO) results in dramatically increased parotid gland protein synthesis, processing and cell proliferation. The authors have shown that in RPAC in vitro, US -AR stimulation has similar effect on protein synthesis and processing. Proto-oncogenes have been implicated in growth regulation, differentiation and in mediating some extracellular stimulated events at the level of gene expression. To understand the regulation of cellular events after US -AR stimulation, the expression of c-fos, c-myc and p53 was investigated. RPAC were incubated with or without 10 VM ISO for 15, 30, 60 min. mRNA was isolated from cells and hybridization analysis was performed on nitrocellulose paper-transferred mRNA using TSP-labeled DNA probes. At early time points, the levels of c-fos gene activation in ISO-treated and control cells were comparable. After 60 min of ISO treatment, a sharp 20-30 fold induction of c-fos expression occurred. Similar increases in c-myc and p53 gene expression were observed after 60 min of ISO treatment. The authors data indicate that early effects of US -AR stimulation of RPAC include induction of c-fos, c-myc and p53 gene expression as well as enhanced protein synthesis and processing.
- Research Organization:
- National Institute of Health, Bethesda, MD
- OSTI ID:
- 6359603
- Report Number(s):
- CONF-870644-; TRN: 87-028679
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
CELL PROLIFERATION
ONCOGENES
GENE REGULATION
PROTEINS
BIOSYNTHESIS
SYMPATHOMIMETICS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
MESSENGER-RNA
PHOSPHORUS 32
RATS
SALIVARY GLANDS
TRACER TECHNIQUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DRUGS
GENES
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
REACTION KINETICS
RNA
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques