Control of c-fos and c-myc proto-oncogene induction in rat thyroid cells in culture

Journal Article · Sun Nov 01 04:00:00 EST 1987 · Molecular Endocrinology; (USA)

DOI:https://doi.org/10.1210/mend-1-11-839· OSTI ID:6318200

Isozaki, O; Kohn, L D ^[1]

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1. National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD (USA)

Removal of TSH, insulin, and cortisol from the medium, and decreasing the serum content to 0.2%, abolishes both the proliferate and differentiated state of FRTL-5 rat thyroid cells in culture. In these basal conditions, the individual addition of TSH, insulin, insulin-like growth factor-I (IGF-I), phorbol 12-myristate 13-acetate (TPA), alpha 1-adrenergic agents, or A23187, increase c-myc and/or c-fos proto-oncogene expression. Under the same conditions, only the addition of TSH increased cAMP levels; 8-bromo-cAMP can increase c-myc or c-fos mRNA levels. Pretreatment of cells with phorbol 12,13-dibutyrate, an agent which down regulates the C-kinase, completely inhibits the effect of TPA on proto-oncogene expression but has no affect on the A23187 induced-increase. The sum of these results indicate that at least four separate signal systems independently increase c-myc or c-fos gene expression in FRTL-5 cells cAMP (TSH), C-kinase (TPA), Ca++/phosphoinositide (A23187), and that influenced by insulin/IGF-I. None of the ligands, when individually returned to cells in basal medium (no TSH, insulin, or cortisol and only 0.2% serum), increases cell number; norepinephrine, and A23187 do not increase (3H)thymidine incorporation into DNA under these conditions; and combinations of the ligands can be more than additive in effecting (3H)thymidine incorporation into DNA but are only additive in effecting proto-oncogene expression. Insulin/IGF-I plus TSH or insulin/IGF-I plus norepinephrine can increase both proto-oncogene expression and (3H)thymidine incorporation into DNA to the same extent; however, the former combination can increase cell number whereas the latter cannot. There is therefore no simple correlation between the ability of the above ligands to increase proto-oncogene expression and their ability to increase cell number or induce DNA synthesis.

• OSTI ID: 6318200
• Journal Information: Molecular Endocrinology; (USA), Journal Name: Molecular Endocrinology; (USA) Vol. 1:11; ISSN 0888-8809; ISSN MOENE
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
BIOLOGICAL EFFECTS
BODY
CARCINOGENS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL PROLIFERATION
DNA
DRUGS
ENDOCRINE GLANDS
ESTERS
GENE REGULATION
GENES
GLANDS
GLOBULINS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
LIGANDS
MAMMALS
MESSENGER-RNA
MITOGENS
NEUROREGULATORS
NORADRENALINE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ONCOGENES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHORBOL ESTERS
PITUITARY HORMONES
PROTEINS
PYRIMIDINES
RATS
RIBOSIDES
RNA
RODENTS
SYMPATHOMIMETICS
THYMIDINE
THYROGLOBULIN
THYROID
THYROID CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TSH
VERTEBRATES