Cerebral acidosis in focal ischemia: I. A method for the simultaneous measurement of local cerebral pH with cerebral glucose utilization or cerebral blood flow in the rat

Journal Article · Mon Dec 01 04:00:00 EST 1986 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1986.122· OSTI ID:6791511

Hakim, A M; Arrieta, M

We developed a technique for the simultaneous autoradiographic determination of local cerebral pH (LCpH) using (/sup 14/C)dimethyloxazolidinedione ((/sup 14/C)DMO) with either local cerebral glucose utilization (LCGU) using (/sup 14/C)deoxyglucose ((/sup 14/C)DG) or local CBF (LCBF) using (/sup 14/C)iodoantipyrine ((/sup 14/C)IAP). The method evolved from the observation that (/sup 14/C)DMO is unstable, which is shown here to be due to sublimation of the compound. Thus, the technique consists of administering to rats (/sup 14/C)DMO followed by either (/sup 14/C)DG or (/sup 14/C)IAP. The first autoradiographic film that results contains both labeled compounds. The brain sections are then exposed in a fume hood until the complete disappearance of (/sup 14/C)DMO, which usually required 10 days. The second film thus contains only the non-DMO isotope administered. Blood levels of the two isotopes are calculated from the total concentrations of isotope and the rate of decline of (/sup 14/C)DMO in blood. This information is then used to determine cerebral pH and the other cerebral function of interest. Using this double-label technique in normal rats, LCpH, LCGU, and LCBF can be determined with the same accuracy as with single-label techniques. In rats with middle cerebral artery occlusion, this technique yielded LCpH and LCBF values 4 h after occlusion that were not statistically different from those obtained by separate determinations. Thus, interactions between LCpH and either LCBF or LCGU can be studied by this technique in normal and pathological conditions.

• Research Organization: McGill Univ., Quebec
• OSTI ID: 6791511
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Journal Name: J. Cereb. Blood Flow Metab.; (United States) Vol. 6; ISSN JCBMD
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
AUTORADIOGRAPHY
BLOOD FLOW
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
DISEASES
DOUBLE LABELLING
GLUCOSE
HEXOSES
ISCHEMIA
LABELLED COMPOUNDS
LABELLING
MAMMALS
MEASURING METHODS
MEDICINE
MONOSACCHARIDES
NERVOUS SYSTEM
NEUROLOGY
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PH VALUE
RATS
RODENTS
SACCHARIDES
VASCULAR DISEASES
VERTEBRATES