A study of the localized humoral immune response to implicated microorganisms in juvenile periodontitis

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:6791124

Hall, E R

A study was undertaken using an in vitro explant culture system to determine the presence of immunoglobulins (IgG, IgA, and IgM) in the supernatant fluids (SF) of disease gingival tissue explant cultures. Studies were also undertaken to determine if the de novo biosynthesis of {sup 14}C-immunoglobulins could be observed in the explant cultures of diseased tissues from juvenile periodontitis (JP) patients. Radiolabeled proteins were detected in the SF and immunodiffusion studies using goat antihuman gamma, alpha or mu chain serum revealed the presence of IgG and IgA but no IgM present in the SF of the JP gingival tissue explant cultures. Immunodiffusion studies using goat anti-human gamma chain serum with Staph protein A isolated IgG fractions of the SF, followed by autoradiography of the IgG precipitation lines demonstrated the biosynthesis of IgG by the JP gingival tissue explant cultures. The serological studies suggested that local immune response in JP was to a polymicrobic infection. The SF of JP showed significantly higher levels of antibody reactivity to B. intermedius, C. ochracea, E. nodatum and P. micros as compared to healthy tissues. The local antibody response to the microorganisms tested differed from that observed in the sera of the patients.

• Research Organization: Maryland Univ., Baltimore, MD (USA)
• OSTI ID: 6791124
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADOLESCENTS
AGE GROUPS
BACTERIA
BACTERIAL DISEASES
BIOSYNTHESIS
CARBON 14 COMPOUNDS
DIGESTIVE SYSTEM
DISEASES
GLOBULINS
IMMUNE REACTIONS
IMMUNOGLOBULINS
IN VITRO
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
ORAL CAVITY
ORGANIC COMPOUNDS
PATHOGENESIS
PROTEINS
SKELETAL DISEASES
SYNTHESIS
TISSUE CULTURES
TRACER TECHNIQUES