Production of immunoglobulins in gingival tissue explant cultures from juvenile periodontitis patients

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Journal of Periodontology; (USA)

DOI:https://doi.org/10.1902/jop.1990.61.10.603· OSTI ID:6068920

Hall, E R; Falkler, Jr, W A; Suzuki, J B ^[1]

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1. Univ. of Maryland Dental School, Baltimore (USA)

B lymphocytes and plasma cells are histologically observed in granulomatous periodontal tissues of juvenile periodontitis (JP) patients. Local immune processes may participate in protective or immunopathologic roles in the pathogenesis of this disease. An in vitro explant culture system was utilized to demonstrate the production of immunoglobulins by diseased JP tissues. Immunodiffusion studies using goat anti-human gamma, alpha, or mu chain serum revealed IgG to be the major immunoglobulin present in 92% of the day 1 supernatant fluids (SF) of the 47 JP gingival tissue explant cultures. IgA was present in 15% of the SF; however, no IgM was detected. Staph Protein A isolated 14C-labeled IgG from the SF, when allowed to react with goat anti-human gamma chain serum, formed lines of precipitation. Positive autoradiographs confirmed the biosynthesis of IgG by the explant cultures. The in vitro gingival tissue explant culture system described provides a useful model for the study of localized immunoglobulins produced by diseased tissues of JP patients.

• OSTI ID: 6068920
• Journal Information: Journal of Periodontology; (USA), Journal Name: Journal of Periodontology; (USA) Vol. 61:10; ISSN 0022-3492; ISSN JOPRA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BIOSYNTHESIS
CARBON 14 COMPOUNDS
DIGESTIVE SYSTEM
GLOBULINS
IMMUNOGLOBULINS
INFLAMMATION
JUVENILES
LABELLED COMPOUNDS
ORAL CAVITY
ORGANIC COMPOUNDS
PATHOGENESIS
PATHOLOGICAL CHANGES
PATIENTS
PROTEINS
SYMPTOMS
SYNTHESIS